INDUCED SPAWNING OF CAPTIVE TRIPLETAIL LOBOTES SURINAMENSIS

<p>The Tripletail, <em>Lobotes surinamensis</em>, is an emerging candidate species for U.S. marine aquaculture. This work aimed to address two bottlenecks for hatchery production of the species by developing a hormonal induction protocol to obtain fertile spawns from captive brooders and a method for sex identification of candidate brooders.</p> <p>Single pairs selected among a captive-held broodstock conditioned under a natural photothermal cycle were induced with one of five treatments (n = 5 or 6 replicates per treatment). Control (no hormone) and hCG (1,100 IU.kg^-1 for females, 550 IU.kg^-1 for males) pairs did not spawn. Pairs treated with GnRHa slow-release implants (75 mg.kg^-1 for females, 55 mg.kg^-1 for males) produced 1 to 2 spawns and, on average 695,899 eggs but fertility was very low (0.58%). Administration of Domperidone at 5 or 10 mg.kg^-1 in conjunction with GnRHa implants improved all metrics with best results obtained in the 10 mg.kg^-1 treatment-group (65.3% fertility, 2.83 spawns following induction, over 1.5 M eggs per mating pair, and larval survival through 4 dph averaging 33.3%). Treatment with GnRHa and Domperidone a week after an initial hCG injection did not improve these results.</p> <p>Plasma levels of 11-Ketotestosterone and Estradiol 17b were assayed in males and females during the spawning season using a competitive ELISA assay. Sex identification was most effective using 11-Ketotestosterone with a 1.19% error rate in cross validation of the training set. Males with low levels of this hormone in the test dataset were mis-identified with a higher rate (7.83%) and may require additional measurements.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Masters Thesis

Year dc:date.available

2022

Author dc:creator

• Adams, Nicholas

Contributors dc:contributor

• Eric Saillant
• Reginald Blaylock
• Frank Hernandez

• LOBOTES SURINAMENSIS
• Tripletail
• Reproduction
• Aquaculture
• ELISA
• Hormones
• Marine Biology

Repository record dc:identifier

https://aquila.usm.edu/masters_theses/895

OAI identifier oai:identifier

oai:aquila.usm.edu:masters_theses-1958