The Tripletail, Lobotes surinamensis, is an emerging candidate species for U.S. marine aquaculture. This work aimed to address two bottlenecks for hatchery production of the species by developing a hormonal induction protocol to obtain fertile spawns from captive brooders and a method for sex identification of candidate brooders.
Single pairs selected among a captive-held broodstock conditioned under a natural photothermal cycle were induced with one of five treatments (n = 5 or 6 replicates per treatment). Control (no hormone) and hCG (1,100 IU.kg-1 for females, 550 IU.kg-1 for males) pairs did not spawn. Pairs treated with GnRHa slow-release implants (75 mg.kg-1 for females, 55 mg.kg-1 for males) produced 1 to 2 spawns and, on average 695,899 eggs but fertility was very low (0.58%). Administration of Domperidone at 5 or 10 mg.kg-1 in conjunction with GnRHa implants improved all metrics with best results obtained in the 10 mg.kg-1 treatment-group (65.3% fertility, 2.83 spawns following induction, over 1.5 M eggs per mating pair, and larval survival through 4 dph averaging 33.3%). Treatment with GnRHa and Domperidone a week after an initial hCG injection did not improve these results.
Plasma levels of 11-Ketotestosterone and Estradiol 17b were assayed in males and females during the spawning season using a competitive ELISA assay. Sex identification was most effective using 11-Ketotestosterone with a 1.19% error rate in cross validation of the training set. Males with low levels of this hormone in the test dataset were mis-identified with a higher rate (7.83%) and may require additional measurements.
","abstract_html":"<p>The Tripletail, <em>Lobotes surinamensis</em>, is an emerging candidate species for U.S. marine aquaculture. This work aimed to address two bottlenecks for hatchery production of the species by developing a hormonal induction protocol to obtain fertile spawns from captive brooders and a method for sex identification of candidate brooders.</p> <p>Single pairs selected among a captive-held broodstock conditioned under a natural photothermal cycle were induced with one of five treatments (n = 5 or 6 replicates per treatment). Control (no hormone) and hCG (1,100 IU.kg<sup>-1</sup> for females, 550 IU.kg<sup>-1</sup> for males) pairs did not spawn. Pairs treated with GnRHa slow-release implants (75 mg.kg<sup>-1</sup> for females, 55 mg.kg<sup>-1</sup> for males) produced 1 to 2 spawns and, on average 695,899 eggs but fertility was very low (0.58%). Administration of Domperidone at 5 or 10 mg.kg<sup>-1</sup> in conjunction with GnRHa implants improved all metrics with best results obtained in the 10 mg.kg<sup>-1</sup> treatment-group (65.3% fertility, 2.83 spawns following induction, over 1.5 M eggs per mating pair, and larval survival through 4 dph averaging 33.3%). Treatment with GnRHa and Domperidone a week after an initial hCG injection did not improve these results.</p> <p>Plasma levels of 11-Ketotestosterone and Estradiol 17b were assayed in males and females during the spawning season using a competitive ELISA assay. Sex identification was most effective using 11-Ketotestosterone with a 1.19% error rate in cross validation of the training set. Males with low levels of this hormone in the test dataset were mis-identified with a higher rate (7.83%) and may require additional measurements.</p>","abstract_has_math":false,"creators":["Adams, Nicholas"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":["Eric Saillant","Reginald Blaylock","Frank Hernandez"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-05-12T07:00:00Z","date_published":"2022-05-12T07:00:00Z","updated_at":"2026-07-24T05:45:33Z","subjects":["LOBOTES SURINAMENSIS","Tripletail","Reproduction","Aquaculture","ELISA","Hormones","Marine Biology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://aquila.usm.edu/masters_theses/895","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Eric Saillant","Reginald Blaylock","Frank Hernandez"]},{"key":"dc:creator","label":"Author","values":["Adams, Nicholas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2024-04-01T07:00:00Z"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["LOBOTES SURINAMENSIS","Tripletail","Reproduction","Aquaculture","ELISA","Hormones","Marine Biology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://aquila.usm.edu/masters_theses/895"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The Tripletail, Lobotes surinamensis, is an emerging candidate species for U.S. marine aquaculture. This work aimed to address two bottlenecks for hatchery production of the species by developing a hormonal induction protocol to obtain fertile spawns from captive brooders and a method for sex identification of candidate brooders.
Single pairs selected among a captive-held broodstock conditioned under a natural photothermal cycle were induced with one of five treatments (n = 5 or 6 replicates per treatment). Control (no hormone) and hCG (1,100 IU.kg-1 for females, 550 IU.kg-1 for males) pairs did not spawn. Pairs treated with GnRHa slow-release implants (75 mg.kg-1 for females, 55 mg.kg-1 for males) produced 1 to 2 spawns and, on average 695,899 eggs but fertility was very low (0.58%). Administration of Domperidone at 5 or 10 mg.kg-1 in conjunction with GnRHa implants improved all metrics with best results obtained in the 10 mg.kg-1 treatment-group (65.3% fertility, 2.83 spawns following induction, over 1.5 M eggs per mating pair, and larval survival through 4 dph averaging 33.3%). Treatment with GnRHa and Domperidone a week after an initial hCG injection did not improve these results.
Plasma levels of 11-Ketotestosterone and Estradiol 17b were assayed in males and females during the spawning season using a competitive ELISA assay. Sex identification was most effective using 11-Ketotestosterone with a 1.19% error rate in cross validation of the training set. Males with low levels of this hormone in the test dataset were mis-identified with a higher rate (7.83%) and may require additional measurements.
"]},{"key":"dc:title","label":"Title","values":["INDUCED SPAWNING OF CAPTIVE TRIPLETAIL LOBOTES SURINAMENSIS"]}]}],"canonical_facts":{"dc:contributor":["Eric Saillant","Reginald Blaylock","Frank Hernandez"],"dc:creator":["Adams, Nicholas"],"dc:date.available":["2024-04-01T07:00:00Z"],"dc:description.abstract":["The Tripletail, Lobotes surinamensis, is an emerging candidate species for U.S. marine aquaculture. This work aimed to address two bottlenecks for hatchery production of the species by developing a hormonal induction protocol to obtain fertile spawns from captive brooders and a method for sex identification of candidate brooders.
Single pairs selected among a captive-held broodstock conditioned under a natural photothermal cycle were induced with one of five treatments (n = 5 or 6 replicates per treatment). Control (no hormone) and hCG (1,100 IU.kg-1 for females, 550 IU.kg-1 for males) pairs did not spawn. Pairs treated with GnRHa slow-release implants (75 mg.kg-1 for females, 55 mg.kg-1 for males) produced 1 to 2 spawns and, on average 695,899 eggs but fertility was very low (0.58%). Administration of Domperidone at 5 or 10 mg.kg-1 in conjunction with GnRHa implants improved all metrics with best results obtained in the 10 mg.kg-1 treatment-group (65.3% fertility, 2.83 spawns following induction, over 1.5 M eggs per mating pair, and larval survival through 4 dph averaging 33.3%). Treatment with GnRHa and Domperidone a week after an initial hCG injection did not improve these results.
Plasma levels of 11-Ketotestosterone and Estradiol 17b were assayed in males and females during the spawning season using a competitive ELISA assay. Sex identification was most effective using 11-Ketotestosterone with a 1.19% error rate in cross validation of the training set. Males with low levels of this hormone in the test dataset were mis-identified with a higher rate (7.83%) and may require additional measurements.
"],"dc:identifier":["https://aquila.usm.edu/masters_theses/895"],"dc:subject":["LOBOTES SURINAMENSIS","Tripletail","Reproduction","Aquaculture","ELISA","Hormones","Marine Biology"],"dc:title":["INDUCED SPAWNING OF CAPTIVE TRIPLETAIL LOBOTES SURINAMENSIS"],"thesis:degree_level":["Masters Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T05:45:33Z"}