Comparison of Supported Liquid Extraction and Solid Phase Extraction of Methamphetamine from Urine and Method Validation by Gas Chromatography-Mass Spectrometry

<p>Applications of SLE (supported liquid extraction) is a relatively new extraction technique that is not widely used in forensic toxicology. SLE is also easier and less time consuming than traditional LLE (liquid-liquid extraction) and SPE (Solid phase extraction). This work will compare the extraction efficiencies between SLE and SPE for methamphetamine from urine with detection and quantitation using GC-MS (gas chromatography- mass spectrometry). The SLE cartridges provide better analyte recoveries, eliminate emulsion formation, and allow for shorter processing time than other extraction techniques. The SPE cartridges used have a mixed-mode non-polar/strong cation exchange retention mechanism to extract basic analytes like MA from urine and other aqueous samples. Optimal retention for SLE was obtained by modifying the urine samples two pH units below the pKa of MA by ensuring the amine groups are properly charged to adhere to the SPE sorbent.</p> <p>Both SPE and SLE calibration range was determined to be 0.5 mg/L to 15 mg/L. MA recovery from urine was better from SPE than SLE with higher accuracy, precision, and extraction efficiency. SPE also demonstrated better linearity. The LOD and LOQ were determined to be 0.25 mg/L for both SLE and SPE. For the extraction of MA from urine, SPE was found to be the preferred method.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Masters Thesis

Year dc:date.available

2021

Author dc:creator

• Hood, Taylor

Contributors dc:contributor

• Dr. Xuyang He
• Dr. Song Guo
• Dr. Wujian Miao

• Methamphetamine
• Urine
• Solid Phase Extraction
• Supported Liquid Extraction
• Gas Chromatography
• Mass Spectrometry
• Analytical Chemistry

Repository record dc:identifier

https://aquila.usm.edu/masters_theses/841

OAI identifier oai:identifier

oai:aquila.usm.edu:masters_theses-1902