Comparison Study Between Post-Fermentation Stillage and Pre-Fermentation Mash Utilizing Acidification and Steam Explosion Techniques for Cellulose Saccharification

<p>Pre-fermentation mash fiber and post-distillation stillage fiber were examined and compared using a variety of preparatory techniques to determine the better source for cellulose fiber saccharification. Once screened, dried, and diluted to a 10% solution, mash fiber and stillage fiber were exposed to increasing temperatures for steam explosion techniques as well as increasing acidification techniques. Both underwent enzymatic saccharification to convert the exposed cellulose to glucose and other sugars. Once the optimum steam explosion technique parameters and acidification parameters were determined to be 2.5% sulfuric acid at 127.8°C for 1 hour, a comparison of the saccharification of pre-fermentation mash fiber and post-distillation stillage fiber under these conditions was conducted. While both are capable sources, post-fermentative stillage provides more fiber (64.18%) that shows approximately 6% greater ability of being degraded than the available fiber content in pre-fermentation mash, which was only 60.92% of the original dried sample.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Masters Thesis

Discipline thesis:degree_discipline

Polymers and High Performance Materials

Year dc:date.available

2017

Author dc:creator

• McKee, Daniel

Contributors dc:contributor

• Sarah Morgan
• James Rawlins
• Jeffrey Wiggins

• Enzymatic Saccharification; Cellulose; Hemicellulose; Acidification; Steam Explosion; Stillage
• Polymer Science

Repository record dc:identifier

https://aquila.usm.edu/masters_theses/323

OAI identifier oai:identifier

oai:aquila.usm.edu:masters_theses-1367