Pre-fermentation mash fiber and post-distillation stillage fiber were examined and compared using a variety of preparatory techniques to determine the better source for cellulose fiber saccharification. Once screened, dried, and diluted to a 10% solution, mash fiber and stillage fiber were exposed to increasing temperatures for steam explosion techniques as well as increasing acidification techniques. Both underwent enzymatic saccharification to convert the exposed cellulose to glucose and other sugars. Once the optimum steam explosion technique parameters and acidification parameters were determined to be 2.5% sulfuric acid at 127.8°C for 1 hour, a comparison of the saccharification of pre-fermentation mash fiber and post-distillation stillage fiber under these conditions was conducted. While both are capable sources, post-fermentative stillage provides more fiber (64.18%) that shows approximately 6% greater ability of being degraded than the available fiber content in pre-fermentation mash, which was only 60.92% of the original dried sample.
","abstract_html":"<p>Pre-fermentation mash fiber and post-distillation stillage fiber were examined and compared using a variety of preparatory techniques to determine the better source for cellulose fiber saccharification. Once screened, dried, and diluted to a 10% solution, mash fiber and stillage fiber were exposed to increasing temperatures for steam explosion techniques as well as increasing acidification techniques. Both underwent enzymatic saccharification to convert the exposed cellulose to glucose and other sugars. Once the optimum steam explosion technique parameters and acidification parameters were determined to be 2.5% sulfuric acid at 127.8°C for 1 hour, a comparison of the saccharification of pre-fermentation mash fiber and post-distillation stillage fiber under these conditions was conducted. While both are capable sources, post-fermentative stillage provides more fiber (64.18%) that shows approximately 6% greater ability of being degraded than the available fiber content in pre-fermentation mash, which was only 60.92% of the original dried sample.</p>","abstract_has_math":false,"creators":["McKee, Daniel"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Masters Thesis","degree_discipline":"Polymers and High Performance Materials","degree_department":null,"school":null,"contributors":["Sarah Morgan","James Rawlins","Jeffrey Wiggins"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-12-01T08:00:00Z","date_published":"2017-12-01T08:00:00Z","updated_at":"2026-07-24T05:44:50Z","subjects":["Enzymatic Saccharification; Cellulose; Hemicellulose; Acidification; Steam Explosion; Stillage","Polymer Science"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://aquila.usm.edu/masters_theses/323","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Sarah Morgan","James Rawlins","Jeffrey Wiggins"]},{"key":"dc:creator","label":"Author","values":["McKee, Daniel"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2017-10-20T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Polymers and High Performance Materials"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Enzymatic Saccharification; Cellulose; Hemicellulose; Acidification; Steam Explosion; Stillage","Polymer Science"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://aquila.usm.edu/masters_theses/323"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Pre-fermentation mash fiber and post-distillation stillage fiber were examined and compared using a variety of preparatory techniques to determine the better source for cellulose fiber saccharification. Once screened, dried, and diluted to a 10% solution, mash fiber and stillage fiber were exposed to increasing temperatures for steam explosion techniques as well as increasing acidification techniques. Both underwent enzymatic saccharification to convert the exposed cellulose to glucose and other sugars. Once the optimum steam explosion technique parameters and acidification parameters were determined to be 2.5% sulfuric acid at 127.8°C for 1 hour, a comparison of the saccharification of pre-fermentation mash fiber and post-distillation stillage fiber under these conditions was conducted. While both are capable sources, post-fermentative stillage provides more fiber (64.18%) that shows approximately 6% greater ability of being degraded than the available fiber content in pre-fermentation mash, which was only 60.92% of the original dried sample.
"]},{"key":"dc:title","label":"Title","values":["Comparison Study Between Post-Fermentation Stillage and Pre-Fermentation Mash Utilizing Acidification and Steam Explosion Techniques for Cellulose Saccharification"]}]}],"canonical_facts":{"dc:contributor":["Sarah Morgan","James Rawlins","Jeffrey Wiggins"],"dc:creator":["McKee, Daniel"],"dc:date.available":["2017-10-20T07:00:00Z"],"dc:description.abstract":["Pre-fermentation mash fiber and post-distillation stillage fiber were examined and compared using a variety of preparatory techniques to determine the better source for cellulose fiber saccharification. Once screened, dried, and diluted to a 10% solution, mash fiber and stillage fiber were exposed to increasing temperatures for steam explosion techniques as well as increasing acidification techniques. Both underwent enzymatic saccharification to convert the exposed cellulose to glucose and other sugars. Once the optimum steam explosion technique parameters and acidification parameters were determined to be 2.5% sulfuric acid at 127.8°C for 1 hour, a comparison of the saccharification of pre-fermentation mash fiber and post-distillation stillage fiber under these conditions was conducted. While both are capable sources, post-fermentative stillage provides more fiber (64.18%) that shows approximately 6% greater ability of being degraded than the available fiber content in pre-fermentation mash, which was only 60.92% of the original dried sample.
"],"dc:identifier":["https://aquila.usm.edu/masters_theses/323"],"dc:subject":["Enzymatic Saccharification; Cellulose; Hemicellulose; Acidification; Steam Explosion; Stillage","Polymer Science"],"dc:title":["Comparison Study Between Post-Fermentation Stillage and Pre-Fermentation Mash Utilizing Acidification and Steam Explosion Techniques for Cellulose Saccharification"],"thesis:degree_discipline":["Polymers and High Performance Materials"],"thesis:degree_level":["Masters Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T05:44:50Z"}