Physical Map between Marker 8O7 and 146O17 on the Medicago truncatula Linkage Group 1 that Contains the NIP Gene

The Medicago truncatula NIP gene is located on M. truncatula Linkage Group 1. Informative recombinants showed crossovers that localize the NIP gene between markers 146O17 and 23C16D. Marker 164N9 co-segregates with the NIP gene, and the location of marker 164N9 is between markers 146O17 and 23C16D. Based upon data from the Medicago genome sequencing project, a subset of the model legume Medicago truncatula bacterial artificial chromosomes (BACs) were used to create a physical map on the DNA in this genetic internal. BACs near the potential NIP gene location near marker 164N9 were identified, and used in experiments to predict the physical map by a BAC-by-BAC strategy. Using marker 164N9 as a center point, and chromosome walking outward, the physical map toward markers 146O17 and 23C16D was built. The chromosome walk consisted of a virtual walk, made with existing sequence of BACs from the Medicago genome project, hybridizations to filters containing BAC DNA, and PCR reactions to confirm that predicted overlapping BACs contained DNA that yielded similar PCR products. In addition, the primers which are made for physical mapping via PCR could be good genetic markers helpful in discovering the location of the NIP gene. As a result of efforts repotted here, gap in physical map between marker 164N9 and 146O17 was closed.

Grantor dc:publisher

University of North Texas

Year dc:date

2007

Author dc:creator

• Lee, Yi-Ching

Contributors dc:contributor

• Dickstein, Rebecca
• Benjamin, Robert C.
• Chapman, Kent D.

• physical map
• NIP gene
• Medicago truncatula
• chromosome walking
• Medicago -- Genome mapping.
• Biochemical markers.

Statement dc:rights

• Public
• Copyright
• Lee, Yi-Ching
• Copyright is held by the author, unless otherwise noted. All rights reserved.

Language dc:language

English

Identifier

oclc: 229101164
https://digital.library.unt.edu/ark:/67531/metadc5152/
ark: ark:/67531/metadc5152

OAI identifier oai:identifier

info:ark/67531/metadc5152