Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"physical map"”.
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Physical Map between Marker 8O7 and 146O17 on the Medicago truncatula Linkage Group 1 that Contains the NIP Gene
… chromosomes (BACs) were used to create a physical map on the DNA in this genetic internal. BACs near the potential NIP gene location near marker 164N9 were identified, and used in experiments to predict the physical map by a BAC-by-BAC strategy. Using marker 164N9 as a center point, and …
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Physical mapping of human chromosome 16 / Sinoula Apostolou.
Involves the construction of a detailed physical map of the distal band of the long arm of human chromosome 16, 16q24.
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The development of tools to allow genetic and genomic analysis of Frankia
… Our approach has focused on the development of a physical and genetic map of the Frankia chromosome by macrorestriction analysis. Agarose-embedded chromosomal DNA plugs were prepared from three Frankia isolates: Eul1c, EAN1pec and Ccl3. The genome sizes of the three strains were determined by …
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Propagation and Restriction Enzyme Mapping of Bacteriophage Pbli Dna
… the order of the fragments were deduced and a physical map was developed.
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Chromosome and Genome Evolution in Culicinae Mosquitoes
… to anchor genomic scaffolds to the cytogenetic map creating a physical map of the genome assembly. We first developed a new gene-based approach for the physical mapping of repeat-rich mosquito genomes. The approach utilized PCR amplification of the DNA probes based on complementary DNA (cDNA) …
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Physical and genetic mapping on mouse proximal chromosome 18
… that cover roughly 15% of the chromosome. The map was assembled based on the presence or absence of 38 DNA microsatellites, from proximal DI8Mit109 through distal D18Mit68. The physical order of those microsatellite STSs have been assigned. The locations of 21 known genes including markers near …
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Testing new genetic and genomic approaches for trait mapping and prediction in wheat (Triticum aestivum) and rice (Oryza spp)
… They have also supported the use of novel mapping approaches, including Multi-parent Advanced Generation Inter-Cross (MAGIC) populations which have increased precision in identifying markers to inform plant breeding practices. In the first part of this thesis, a high density physical map …
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PHYSICAL MAPPING OF THE ORIGINS OF REPLICATION OF BACILLUS SUBTILIS BACTERIOPHAGE SP01
… to localize the origins of replication on the physical map of SP01. The fragments that were consistently detected in this manner correspond to a region of the DNA extending from the middle of the left terminal repeat to about 32 kilobasepairs from the left end of the genome. Thus there is at …
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Applications of Interactive Topographic Maps: Tangibility with Improved Spatial Awareness and Readability
Traditional flat topographic maps are difficult to understand due to the distortion and compromise of the 3-dimensional (3D) spatial representation when it is folded into lower-dimension media (e.g. 2D). During the process, the x-y coordinate of a location can be captured but its physical elevation …
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Isolation and characterization of motility-defective mutants of Haloferax volcanii.
… is a motile extreme halophile, and a detailed physical map of its genome exists. An effort is underway to locate more genes and to increase the detail of this map. Flagellated motile bacteria possess a number of filaments that function as cellular propellers. Motile bacteria swim outward in a …
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Understanding the genetic and physiological control of pre-harvest sprouting and pre-maturity amylase in UK wheat
… the effects of these QTL, as well as to fine-map the QTL with the most significant effect. To achieve these aims, isogenic materials were developed to independently study these QTL effects. Physiological characterisation of these QTL showed that they exert their effects by affecting the …
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Refinement of the physical and genetic maps of the MEN2A region in pericentromeric chromosome 10
… D10S94 , RET, D10S97, and D1OS102. Meiotic mapping in the MEN2A region is limited by a paucity of critical crossovers, attributable in part to reduced rates of recombination in the region, particularly in male meioses. Additional approaches for mapping loci in the pericentromeric region of …
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Cloning and Characterization of the dominant Inhibitor of Wax 1 (Iw1) gene in polyploid wheat
… and biochemical methods. Here, we have fine-mapped Iw1 to a 0.42-cM interval on the short arm of chromosome 2B and we have constructed a physical map, which is currently 1,200 kb in size. Gene models were predicted in silico and we have begun to test candidate genes using allelic diversity …
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Characterization of chalcone synthase genes in red raspberry cultivar (rubus idaeus)
… sources for validating the BAC sequences, and physical map drafts provided better information of this chromosomal segment in this thesis, as they will be useful available tools for further studying in red raspberry genome.
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Physikalische Kartierung der RT1-C/M-Region des Haupthistokompatibilitätskomplexes der Ratte
… clones encompassing 2 Mb, and present a physical map of this MHC region. Forty-five class I exon 4-hybridizing BamHI fragments were detected, including the previously known rat class I genes RT1-E, RT-BM1, RT1-M2, RT1-M3, and RT1-M4. Fifteen- non-class I genes known to map to the …
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Application of Chromosome Mapping to Understanding Evolutionary History of Anopheles Species
… previously. Finally, for the first time a physical map of 12 microsatellite markers for the Asian malaria vector An. stephensi was developed. Knowledge about the chromosomal position of microsatellites was shown to be important for a proper estimation of population genetic parameters. In …
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Comparative genomics of chromosomal rearrangements in malaria mosquitoes
… dynamics of chromosomal inversions, a physical map for an Asian malaria vector, Anopheles stephensi, was created and compared with the maps of the major African malaria vectors A. gambiae and A. funestus No interchromosomal transposition was observed between A. gambiae and A. stephensi. …
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Investigating the genome complexity of Streptomyces clavuligerus
… chromosomal fragments in order to establish the physical map of the S. clavuligerus genome.;Furthermore, to establish a foundation on which the genomic analyses of industrial strains of this species could be carried out, we closed the genome sequence of the S. clavuligerus type strain DSM 738, …
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Molecular Studies of KpnAI and KpnBI R-M Systems : in KLEBSIELLA PNEUMONIAE M5a1 and GM236
… on a 3.6 kb <em>Sau3</em>Al fragment. To map the gene, the <em>hsdR</em><sub>KpnAI</sub> clone was hybridized to a membrane which contained pulsed-field gel electrophoresed fragments of the chromosome of the strain M5al. The <em>hsdR</em><sub>KpnAI </sub>clone was hybridized to a 19 kb …
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Mapping phenotypic traits in swine
… population. For the first analysis, a linkage map consisting of 137 microsatellite markers was used to perform a regression interval analysis with QTLexpress, a publicly available web-based software. In the second analysis, the SOLAR software package was used to perform a variance component …
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