Concentration-dependent Effects of D-Methylphenidate on Frontal Cortex and Spinal Cord Networks in vitro

Spontaneously active frontal cortex and spinal cord networks grown on microelectrode arrays were used to study effects of D-methylphenidate. These central nervous system tissues have relatively low concentrations of dopaminergic and noradrenergic neurons compared to the richly populated loci, yet exhibit similar neurophysiological responses to methylphenidate. The spontaneous spike activity of both tissues was inhibited in a concentration-dependent manner by serial additions of 1-500 µM methylphenidate. Methylphenidate is non-toxic as spike inhibition was recovered following washes. The average concentrations for 50% spike rate inhibition (IC50 ± SD) were 118 ± 52 (n= 6) and 57 ± 43 (n = 11) for frontal cortex and spinal cord networks, respectively. A 3 hour exposure of a network to 1 mM methylphenidate was nontoxic. The effective concentrations described in this study are within the therapeutic dosage range. Therefore, the platform may be used for further investigations of drug mechanisms.

Grantor dc:publisher

University of North Texas

Year dc:date

2004

Author dc:creator

• Miller, Benjamin R.

Contributors dc:contributor

• Gross, Guenter W.
• Fuchs, Jannon L.
• Gopal, Kamakshi V.

• Neural networks (Neurobiology)
• Methylphenidate.
• microelectrode arrays
• D-methylphenidate
• dopamine
• norepinephrine
• neuronal networks

Statement dc:rights

• Public
• Copyright
• Miller, Benjamin R.
• Copyright is held by the author, unless otherwise noted. All rights reserved.

Language dc:language

English

Identifier

oclc: 58796402
https://digital.library.unt.edu/ark:/67531/metadc4689/
ark: ark:/67531/metadc4689

OAI identifier oai:identifier

info:ark/67531/metadc4689