Use of luminescence energy transfer probes to detect genetic variants.

The purpose of this research was to study the hybridization of molecular beacons under different conditions and designs. Data collected suggest that the inconsistency found in the emission intensity of several of these probes may be caused by 3 important factors: length of the probe, nucleotide sequence and, the formation of an alternative complex structure such as a dimer. Of all three factors, dimer formation is the most troublesome, since it reduces the emission of the reporter molecules. A new probe design was used to reduce dimer formation. The emission signal of the improved probe was several folds stronger than those probes with the early design. In this research, dimer formation is detected, furthermore a new probe with a different design was tested. If dimer formation can be reduced molecular beacons can be integrated into more complex hybridization systems providing an important tool in research and diagnosis of genetic disorders.

Grantor dc:publisher

University of North Texas

Year dc:date

2004

Author dc:creator

• Vaccaro, Carlos

Contributors dc:contributor

• Root, Douglas D.
• Dickstein, Rebecca
• Tam, David

• Molecular genetics.
• genetic variant
• FRET
• molecular beacons
• probe design
• emission intensity

Statement dc:rights

• Public
• Copyright
• Vaccaro, Carlos
• Copyright is held by the author, unless otherwise noted. All rights reserved.

Language dc:language

English

Identifier

oclc: 56698652
https://digital.library.unt.edu/ark:/67531/metadc4566/
ark: ark:/67531/metadc4566

OAI identifier oai:identifier

info:ark/67531/metadc4566