{"id":{"repo_id":"unt","oai_identifier":"info:ark/67531/metadc4566"},"canonical_url":"https://search.dev.ndltd.org/etd/unt/info:ark/67531/metadc4566","repository":{"repo_id":"unt","name":"University of North Texas","base_url":"https://digital.library.unt.edu/oai/"},"display":{"title":"Use of luminescence energy transfer probes to detect genetic variants.","abstract":"The purpose of this research was to study the hybridization of molecular beacons under different conditions and designs. Data collected suggest that the inconsistency found in the emission intensity of several of these probes may be caused by 3 important factors: length of the probe, nucleotide sequence and, the formation of an alternative complex structure such as a dimer. Of all three factors, dimer formation is the most troublesome, since it reduces the emission of the reporter molecules. A new probe design was used to reduce dimer formation. The emission signal of the improved probe was several folds stronger than those probes with the early design. In this research, dimer formation is detected, furthermore a new probe with a different design was tested. If dimer formation can be reduced molecular beacons can be integrated into more complex hybridization systems providing an important tool in research and diagnosis of genetic disorders.","abstract_html":"The purpose of this research was to study the hybridization of molecular beacons under different conditions and designs. Data collected suggest that the inconsistency found in the emission intensity of several of these probes may be caused by 3 important factors: length of the probe, nucleotide sequence and, the formation of an alternative complex structure such as a dimer. Of all three factors, dimer formation is the most troublesome, since it reduces the emission of the reporter molecules. A new probe design was used to reduce dimer formation. The emission signal of the improved probe was several folds stronger than those probes with the early design. In this research, dimer formation is detected, furthermore a new probe with a different design was tested. If dimer formation can be reduced molecular beacons can be integrated into more complex hybridization systems providing an important tool in research and diagnosis of genetic disorders.","abstract_has_math":false,"creators":["Vaccaro, Carlos"],"institution":"University of North Texas","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Root, Douglas D.","Dickstein, Rebecca","Tam, David"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2004,"date_issued":"2004-08","date_published":"2004-08","updated_at":"2026-07-24T05:35:09Z","subjects":["Molecular genetics.","genetic variant","FRET","molecular beacons","probe design","emission intensity"],"languages":["English"],"rights":["Public","Copyright","Vaccaro, Carlos","Copyright is held by the author, unless otherwise noted. All rights reserved."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oclc: 56698652","https://digital.library.unt.edu/ark:/67531/metadc4566/","ark: ark:/67531/metadc4566"],"render_values":[{"text":"oclc: 56698652","href":null,"code":true},{"text":"https://digital.library.unt.edu/ark:/67531/metadc4566/","href":"https://digital.library.unt.edu/ark:/67531/metadc4566/","code":true},{"text":"ark: ark:/67531/metadc4566","href":null,"code":true}]}]},"links":{"outbound_url":"https://doi.org/10.12794/metadc4566","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Root, Douglas D.","Dickstein, Rebecca","Tam, David"]},{"key":"dc:creator","label":"Author","values":["Vaccaro, Carlos"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2004-08"]},{"key":"dc:publisher","label":"Institution","values":["University of North Texas"]},{"key":"dc:type","label":"Dc Type","values":["Thesis or Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Molecular genetics.","genetic variant","FRET","molecular beacons","probe design","emission intensity"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["Public","Copyright","Vaccaro, Carlos","Copyright is held by the author, unless otherwise noted. All rights reserved."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oclc: 56698652","doi: 10.12794/metadc4566","https://digital.library.unt.edu/ark:/67531/metadc4566/","ark: ark:/67531/metadc4566"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The purpose of this research was to study the hybridization of molecular beacons under different conditions and designs. Data collected suggest that the inconsistency found in the emission intensity of several of these probes may be caused by 3 important factors: length of the probe, nucleotide sequence and, the formation of an alternative complex structure such as a dimer. Of all three factors, dimer formation is the most troublesome, since it reduces the emission of the reporter molecules. A new probe design was used to reduce dimer formation. The emission signal of the improved probe was several folds stronger than those probes with the early design. In this research, dimer formation is detected, furthermore a new probe with a different design was tested. If dimer formation can be reduced molecular beacons can be integrated into more complex hybridization systems providing an important tool in research and diagnosis of genetic disorders."]},{"key":"dc:format","label":"Dc Format","values":["Text"]},{"key":"dc:title","label":"Title","values":["Use of luminescence energy transfer probes to detect genetic variants."]}]}],"canonical_facts":{"dc:contributor":["Root, Douglas D.","Dickstein, Rebecca","Tam, David"],"dc:creator":["Vaccaro, Carlos"],"dc:date":["2004-08"],"dc:description":["The purpose of this research was to study the hybridization of molecular beacons under different conditions and designs. Data collected suggest that the inconsistency found in the emission intensity of several of these probes may be caused by 3 important factors: length of the probe, nucleotide sequence and, the formation of an alternative complex structure such as a dimer. Of all three factors, dimer formation is the most troublesome, since it reduces the emission of the reporter molecules. A new probe design was used to reduce dimer formation. The emission signal of the improved probe was several folds stronger than those probes with the early design. In this research, dimer formation is detected, furthermore a new probe with a different design was tested. If dimer formation can be reduced molecular beacons can be integrated into more complex hybridization systems providing an important tool in research and diagnosis of genetic disorders."],"dc:format":["Text"],"dc:identifier":["oclc: 56698652","doi: 10.12794/metadc4566","https://digital.library.unt.edu/ark:/67531/metadc4566/","ark: ark:/67531/metadc4566"],"dc:language":["English"],"dc:publisher":["University of North Texas"],"dc:rights":["Public","Copyright","Vaccaro, Carlos","Copyright is held by the author, unless otherwise noted. All rights reserved."],"dc:subject":["Molecular genetics.","genetic variant","FRET","molecular beacons","probe design","emission intensity"],"dc:title":["Use of luminescence energy transfer probes to detect genetic variants."],"dc:type":["Thesis or Dissertation"]},"updated_at":"2026-07-24T05:35:09Z"}