Cassette Systems for Creating Intergeneric Hybrid ATCases

Cassette systems for creating intergeneric hybrid ATCases were constructed. An MluI restriction enzyme site was introduced at the carbamoylphosphate binding site within the pyrB genes of both Pseudomonas putida and Escherichia coli. Two hybrids, E. coli pyrB polar domain fused with P. putida pyrB equatorial domain and P. putida pyrB polar domain fused with E. coli pyrB equatorial domain, are possible. The intergeneric E. coli-P. putida hybrid pyrB gene was constructed and found to encode an active ATCase which complemented an E. coli Pyr- strain. These hybrids are useful for kinetic and expression studies of ATCase in E. coli.

Grantor dc:publisher

University of North Texas

Year dc:date

1999

Author dc:creator

• Simpson, Luci N.

Contributors dc:contributor

• O'Donovan, Gerard A.
• Benjamin, Robert C.
• Farinha, Mark A.

• Escherichia coli -- Genetics.
• Pseudomonas putida -- Genetics.
• Pyrimidine nucleotides -- Metabolism.
• intergeneric hybrid
• ATCase
• genetic research
• hybrid genes

Statement dc:rights

• Public
• Copyright
• Simpson, Luci N.
• Copyright is held by the author, unless otherwise noted. All rights reserved.

Language dc:language

English

Identifier

oclc: 47144653
untcat: b2300276
https://digital.library.unt.edu/ark:/67531/metadc2237/
ark: ark:/67531/metadc2237

OAI identifier oai:identifier

info:ark/67531/metadc2237