{"id":{"repo_id":"unt","oai_identifier":"info:ark/67531/metadc2237"},"canonical_url":"https://search.dev.ndltd.org/etd/unt/info:ark/67531/metadc2237","repository":{"repo_id":"unt","name":"University of North Texas","base_url":"https://digital.library.unt.edu/oai/"},"display":{"title":"Cassette Systems for Creating Intergeneric Hybrid ATCases","abstract":"Cassette systems for creating intergeneric hybrid ATCases were constructed. An MluI restriction enzyme site was introduced at the carbamoylphosphate binding site within the pyrB genes of both Pseudomonas putida and Escherichia coli. Two hybrids, E. coli pyrB polar domain fused with P. putida pyrB equatorial domain and P. putida pyrB polar domain fused with E. coli pyrB equatorial domain, are possible. The intergeneric E. coli-P. putida hybrid pyrB gene was constructed and found to encode an active ATCase which complemented an E. coli Pyr- strain. These hybrids are useful for kinetic and expression studies of ATCase in E. coli.","abstract_html":"Cassette systems for creating intergeneric hybrid ATCases were constructed. An MluI restriction enzyme site was introduced at the carbamoylphosphate binding site within the pyrB genes of both Pseudomonas putida and Escherichia coli. Two hybrids, E. coli pyrB polar domain fused with P. putida pyrB equatorial domain and P. putida pyrB polar domain fused with E. coli pyrB equatorial domain, are possible. The intergeneric E. coli-P. putida hybrid pyrB gene was constructed and found to encode an active ATCase which complemented an E. coli Pyr- strain. These hybrids are useful for kinetic and expression studies of ATCase in E. coli.","abstract_has_math":false,"creators":["Simpson, Luci N."],"institution":"University of North Texas","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["O'Donovan, Gerard A.","Benjamin, Robert C.","Farinha, Mark A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1999,"date_issued":"1999-12","date_published":"1999-12","updated_at":"2026-07-24T05:34:52Z","subjects":["Escherichia coli -- Genetics.","Pseudomonas putida -- Genetics.","Pyrimidine nucleotides -- Metabolism.","intergeneric hybrid","ATCase","genetic research","hybrid genes"],"languages":["English"],"rights":["Public","Copyright","Simpson, Luci N.","Copyright is held by the author, unless otherwise noted. 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