Large Scale Cross-Linking Mass Spectrometry as the Missing Link in the Systems Biology Toolbox

The function of a protein is mediated by its ability to form precise three-dimensional structures or specific protein-protein interaction (PPI) interfaces. However, it has been challenging to study these aspects of the proteome at scale, in sufficient detail, and in a generalisable way. Cross-linking mass spectrometry (XL-MS) performed on high complexity samples (such as entire organelles or cells) theoretically enables the large scale mapping and monitoring of protein structural conformations and PPI interfaces in native proteoforms. However, as the large scale XL-MS field is young and historically driven by the reporting of technological innovations, assessment of the biological accuracy (and overall utility) of large scale XL-MS studies is still largely underexplored. This thesis applies large scale XL-MS to two eukaryotic systems (Saccharomyces cerevisiae and cultured human cells) and is presented as three independent studies. Firstly, it reports the first budding yeast XL-MS interactome, generated by cross-linking intact nuclei isolated from wild-type cells with DSSO. The wealth of yeast interactome data enabled identification of an inflated false discovery rate for cross-links representing PPIs, and development of a novel quality control approach. Surprisingly, the high confidence PPIs were substantially orthogonal to historical binary interactome mapping efforts. Subsequently, it reports the largest XL-MS dataset currently available for any species, generated by diversifying analyses (cross-linkers, mass spectrometry analyses, software) performed on organelles isolated from human HEK293 cells. Here, we show how the ~30,000 low-resolution distance constraints generated in near-native proteoforms can be used to uniquely (1) contextualise regions within existing experimental protein structures, (2) annotate interfaces in PPIs and multi-protein complexes, and (3) validate structures predicted by computational modellers like AlphaFold. Finally, this thesis reports the use of quantitative XL-MS to explore links between gene function and cellular phenotype, comparing wild-type yeast with a highly pleiotropic deletion strain (Δhpm1) using SILAC multiplexing and PIR cross-linking of intact cells. The untargeted comparison of structural conformations and PPIs uniquely enabled insights into phenotypes entirely invisible to investigation of protein abundance alone. This thesis will therefore argue that XL-MS is the missing link in the systems biology toolbox.

Grantor dc:publisher

UNSW, Sydney

Year dc:date

2022

Author dc:creator

• Bartolec-Criss, Tara ; https://orcid.org/0000-0002-5291-6972

• proteomics
• cross-linking mass spectrometry
• protein structure
• protein-protein interactions
• interactomics
• anzsrc-for: 310205 Proteomics and metabolomics
• anzsrc-for: 310112 Structural biology (incl. macromolecular modelling)
• anzsrc-for: 310109 Proteomics and intermolecular interactions (excl. medical proteomics)

Statement dc:rights

• open access
• CC BY 4.0
• free_to_read

Licence

• https://purl.org/coar/access_right/c_abf2
• https://creativecommons.org/licenses/by/4.0/

Language dc:language

en

Identifier

https://doi.org/10.26190/unsworks/24515

OAI identifier oai:identifier

oai:unsworks.library.unsw.edu.au:1959.4/100808