Testing the Role of Cyclic Nucleotides in Regulating Ion Channels Essential to Pollen Fertility

It is unknown if plant cyclic nucleotide gated channels (CNGCs) are activated by cyclic nucleotide monophosphates (cNMPs, e.g. cAMP or cGMP), or even if cNMPs are present at physiologically significant levels in plants. cNMP presence in growing A.thaliana pollen tubes was visualized using fluorescent biosensors, and a competitive binding assay was designed to gauge CNGC binding affinity for cNMPs. cAMP- and cGMP- specific biosensors showed strong, uniform fluorescence throughout pollen tube; the competitive binding assay was not completed due to laboratory shutdown

Name thesis:degree_name

Biochemistry and Molecular Biology

Level thesis:degree_level

Honors Thesis

Grantor

University of Nevada, Reno

Year dc:date.issued

2020

Author dc:creator

• Boren, Duncan

Advisor dc:contributor.advisor

• Harper, Jeffrey F.

Committee member dc:contributor.committeemember

• Wiegand, Chrystle

Statement dc:rights

• Creative Commons Attribution 4.0 United States

Language dc:language.iso

en_US, English

Handle dc:identifier.uri

http://hdl.handle.net/11714/7527

OAI identifier oai:identifier

oai:scholarwolf.unr.edu:11714/7527