{"id":{"repo_id":"unr","oai_identifier":"oai:scholarwolf.unr.edu:11714/7527"},"canonical_url":"https://search.dev.ndltd.org/etd/unr/oai:scholarwolf.unr.edu:11714/7527","repository":{"repo_id":"unr","name":"University of Nevada - Reno","base_url":"https://scholarwolf.unr.edu/server/oai/request"},"display":{"title":"Testing the Role of Cyclic Nucleotides in Regulating Ion Channels Essential to Pollen Fertility","abstract":"It is unknown if plant cyclic nucleotide gated channels (CNGCs) are activated by cyclic nucleotide monophosphates (cNMPs, e.g. cAMP or cGMP), or even if cNMPs are present at physiologically significant levels in plants. cNMP presence in growing A.thaliana pollen tubes was visualized using fluorescent biosensors, and a competitive binding assay was designed to gauge CNGC binding affinity for cNMPs. cAMP- and cGMP- specific biosensors showed strong, uniform fluorescence throughout pollen tube; the competitive binding assay was not completed due to laboratory shutdown","abstract_html":"It is unknown if plant cyclic nucleotide gated channels (CNGCs) are activated by cyclic nucleotide monophosphates (cNMPs, e.g. cAMP or cGMP), or even if cNMPs are present at physiologically significant levels in plants. cNMP presence in growing A.thaliana pollen tubes was visualized using fluorescent biosensors, and a competitive binding assay was designed to gauge CNGC binding affinity for cNMPs. cAMP- and cGMP- specific biosensors showed strong, uniform fluorescence throughout pollen tube; the competitive binding assay was not completed due to laboratory shutdown","abstract_has_math":false,"creators":["Boren, Duncan"],"institution":"University of Nevada, Reno","degree_name":"Biochemistry and Molecular Biology","degree_level":"Honors Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Harper, Jeffrey F."],"committee_chairs":[],"committee_members":["Wiegand, Chrystle"],"year":2020,"date_issued":"2020-05-01","date_published":"2020-05-01","updated_at":"2026-07-27T21:47:25Z","subjects":[],"languages":["en_US","English"],"rights":["Creative Commons Attribution 4.0 United States"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11714/7527","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Harper, Jeffrey F."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Wiegand, Chrystle"]},{"key":"dc:creator","label":"Author","values":["Boren, Duncan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2020-07-21T16:46:03Z"]},{"key":"dc:date.issued","label":"Date","values":["2020-05-01"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Honors Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Biochemistry and Molecular Biology"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Nevada, Reno"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]},{"key":"dc:rights","label":"Dc Rights","values":["Creative Commons Attribution 4.0 United States"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11714/7527"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The University of Nevada, Reno Libraries will promptly respond to removal requests related to content that violates intellectual property laws, data protections, or has been uploaded without creator consent. Takedown notices should be directed to our ScholarWolf team (scholarwolf@library.unr.edu) with information about the object, including its full URL and the nature of your complaint."]},{"key":"dc:description.abstract","label":"Abstract","values":["It is unknown if plant cyclic nucleotide gated channels (CNGCs) are activated by cyclic nucleotide monophosphates (cNMPs, e.g. cAMP or cGMP), or even if cNMPs are present at physiologically significant levels in plants. cNMP presence in growing A.thaliana pollen tubes was visualized using fluorescent biosensors, and a competitive binding assay was designed to gauge CNGC binding affinity for cNMPs. cAMP- and cGMP- specific biosensors showed strong, uniform fluorescence throughout pollen tube; the competitive binding assay was not completed due to laboratory shutdown"]},{"key":"dc:format","label":"Dc Format","values":["PDF"]},{"key":"dc:title","label":"Title","values":["Testing the Role of Cyclic Nucleotides in Regulating Ion Channels Essential to Pollen Fertility"]}]}],"canonical_facts":{"dc:contributor.advisor":["Harper, Jeffrey F."],"dc:contributor.committeemember":["Wiegand, Chrystle"],"dc:creator":["Boren, Duncan"],"dc:date.accessioned":["2020-07-21T16:46:03Z"],"dc:date.issued":["2020-05-01"],"dc:description":["The University of Nevada, Reno Libraries will promptly respond to removal requests related to content that violates intellectual property laws, data protections, or has been uploaded without creator consent. Takedown notices should be directed to our ScholarWolf team (scholarwolf@library.unr.edu) with information about the object, including its full URL and the nature of your complaint."],"dc:description.abstract":["It is unknown if plant cyclic nucleotide gated channels (CNGCs) are activated by cyclic nucleotide monophosphates (cNMPs, e.g. cAMP or cGMP), or even if cNMPs are present at physiologically significant levels in plants. cNMP presence in growing A.thaliana pollen tubes was visualized using fluorescent biosensors, and a competitive binding assay was designed to gauge CNGC binding affinity for cNMPs. cAMP- and cGMP- specific biosensors showed strong, uniform fluorescence throughout pollen tube; the competitive binding assay was not completed due to laboratory shutdown"],"dc:format":["PDF"],"dc:identifier.uri":["http://hdl.handle.net/11714/7527"],"dc:language":["English"],"dc:language.iso":["en_US"],"dc:rights":["Creative Commons Attribution 4.0 United States"],"dc:title":["Testing the Role of Cyclic Nucleotides in Regulating Ion Channels Essential to Pollen Fertility"],"dc:type":["Thesis"],"thesis:degree_level":["Honors Thesis"],"thesis:degree_name":["Biochemistry and Molecular Biology"],"thesis:institution_name":["University of Nevada, Reno"]},"updated_at":"2026-07-27T21:47:25Z"}