Localization and Mutational Analysis of the Nuclear and Aggregation-Prone Ime4 Protein in Saccharomyces cerevisiae

<p>In <em>Saccharomyces cerevisiae, </em>Ime4 is a protein that is induced during meiosis and has a primary role in regulating sporulation in starving diploids. One function of Ime4 is methylation of adenosine residues within mRNA transcripts. Recent studies have shown Ime4 to be induced in haploids during the mating response, although its role in mating has not been determined. In this report, I identify the subcellular localization of Ime4 during the mating response through treatment with alpha factor. A plasmid containing IME4-GFP under the control of the medium strength promoter CYC1 was created in order to express the protein in a controlled manner. Lastly, mutational analysis was conducted to determine which regions of the protein were necessary for its nuclear localization, aggregation, and sporulation function.</p>

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biological Sciences

Year

2012

Author dc:creator

• Dehon, Patricia M

Contributors dc:contributor

• Dr. Mary Clancy
• Dr. Wendy Schluchter
• Dr. Zhengchang Liu

• Ime4
• meiosis
• sporulation
• methylation
• aggregation
• nuclear
• Molecular Genetics

Repository record dc:identifier

https://scholarworks.uno.edu/td/1567

OAI identifier oai:identifier

oai:scholarworks.uno.edu:td-2561