Engineering Saccharomyces ceresisiae for the Secretion of an Extracellular Lipase

<p>Developing microbial systems capable of converting low cost lipids into value added products depends on the ability to acquire substrates from the growth media. Saccharomyces cerevisiae can acquire free fatty acids from the growth media and a portion of these lipids can be converted into new lipid products. However, they cannot acquire complex lipids from the growth media unless a nonspecific lipase is included. To circumvent lipase addition, we are genetically engineering S. cerevisiae to secrete a lipase into the growth media. We selected the LIP2 gene from Yarrowia lipolytica, which encodes a nonspecific lipase. Several modifications were made to the LIP2 gene to improve processing. Results identified strains secreting the most lipase. From these results, high producing strains were inserted into an oil inducible vector. Halo assays confirmed lipase secretion, while measuring the fatty acid composition confirmed triacylglycerol breakdown, and yeast uptake of the free fatty acids released.</p>

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biological Sciences

Year

2007

Author dc:creator

• Stewart, Gaynelle

Contributors dc:contributor

• Clancy, Mary
• Timpte, Candace
• Burt-Utley, Kathleen

• Metabolic Engineering
• Saccharomyces cerevisiae
• lipase
• yeast
• carboxypeptidase Y
• KEX2 protease
• codon optimization
• fatty acid methyl esters

Repository record dc:identifier

https://scholarworks.uno.edu/td/577

OAI identifier oai:identifier

oai:scholarworks.uno.edu:td-1577