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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"codon optimization"”.
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Computational Targeted Codon Optimization and Translation with Deep Learning
Codon optimization—the task of recoding a protein’s underlying DNA sequence to maximize expression in a target organism—is a complicated biological optimization problem. Each gene brings a dynamic combination of local and long-range dependencies along with globally imposed constraints specific to …
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Oligonucleotide design and codon optimization for PCR-based gene synthesis
… If the primary concern is a protein sequence, codons can be changed to synonymous codons with little consequence, making it possible to remove problematic repetitive elements. We show that our algorithm can theoretically be extended and used with constraint optimization algorithms to solve the …
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The Role of Codon Usage in Regulating Protein Expression, Structure and Function
Codon usage bias has been observed in the genomes of almost all organisms and is thought to result from selection for efficient translation of highly expressed genes. Many genes, however, exhibit little codon usage bias. It's not clear whether the lack of codon bias for a gene is due to lack of …
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Expression of HIV-1 subtype C nef in E. coli and Nicotiana benthamiana : development of plant-based vaccines for HIV
… anti Nef antiserum raised in sheep. The role of codon optimization in expression of Nef in benthamiana was investigated. A synthetic nef gene was constructed based on the codon usage of benthamiana. The plant codon optimized gene and the wild type nef genes were inserted into the TMV -based …
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Pre-Clinical Development Of Aav Mediated Gene Therapy For Familial Lecithin Cholesterol Acyltransferase Deficiency
… 5e10 GC per kg. Additionally, we expressed four codon optimized variants of human LCAT in vivo using AAV8. Codon optimization had little effect on the efficacy of hLCAT in mice, with only one variant, AAV-TBG-hLCAT-v1, inducing greater increases in HDL-C than WT hLCAT. Lastly, we were able to …
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Large-scale production and characterization of an engineered human olfactory receptor
… PCR-based gene synthesis, which facilitated codon optimization and attachment of different affinity tags for purification. Established methods for the production and purification of rhodopsin were adapted to olfactory receptors through extensive optimization (including a full-spectrum …
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Study of the aryl hydrocarbon receptor as a target for rational drug design
… expression system was constructed expressing codon optimized human full length AhR. This codon optimization is necessary for overexpression of huAhR. RT-PCR data showed that the codon optimized mRNA was more stably expressed than wild types. Overexpressed huAhR protein was degraded by …
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Systems Metabolic Engineering of Microbial Cell Factories for the Synthesis of Value-added Chemicals
… three strategies:: i) cofactor balancing,: ii) codon optimization, and: iii) process optimization. The engineered <italic>Synechocystis</italic> 6803 produced 2.2 g/L D-lactate under photoautotrophic conditions with acetate, the highest reported lactate titer among all known cyanobacterial …
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Optimizing nuclear transgene expression and genome editing for high-value compound production in the green microalga Chlamydomonas reinhardtii
… and using expression-enhancing techniques like codon optimization and intron spreading supports elevating transgene expression rates and therefore production. Additional establishment of targeted genome editing makes these approaches more feasible, however, present transgene silencing and lack …
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Engineering Saccharomyces ceresisiae for the Secretion of an Extracellular Lipase
<p>Developing microbial systems capable of converting low cost lipids into value added products depends on the ability to acquire substrates from the growth media. Saccharomyces cerevisiae can acquire free fatty acids from the growth media and a portion of these lipids can be converted into new …
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PCR-based Synthesis of Codon Optimized cry2Aa Gene for Production of Shoot and Fruit Borer (Leucinodes orbonalis) Resistant Eggplant (Solanum melongena L.) Cultivars
… was to develop eggplant cultivars that express a codon-optimized cry2Aa gene, the sequence of which is based on that of an Indian isolate of Bt, with the eventual goal of producing fully resistant cultivars. The cry2Aa gene was modified for optimal expression in eggplant using the codon usage …
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Gene Expression Control for Synthetic Patterning of Bacterial Populations and Plants
… expression control in the liverwort: I analyzed codon usage across the transcriptome of M. polymorpha, and used insights gained to design codon-optimized fluorescent reporters successfully expressed from its nuclear and chloroplast genomes. For targeting of bacteriophage T7 RNA polymerase to …