Effects of Chronic Sleep and Food Deprivation on In Vivo Levels of Prepro-Hypocretin (PPH)

The hypocretin peptides are two hypothalamic peptides known to be involved in both sleeping and feeding behavior, however their specific roles in these domains are not well understood. The present study sought to determine the effect of chronic (72-hour) sleep deprivation and (48-hour) food deprivation on preprohypocretin (PPH), which is the precursor for the hypocretin peptides. PPH levels were visualized and quantified via in situ hybridization. A three-factor ANOVA ( group x dorsal/ventral x medial/lateral) revealed a significant effect of subregion, specifically dorsal/middle and ventral/medial exhibited elevated PPH levels, however there was no effect of group. A between group one-way ANOVA revealed no effect of group on PPH levels. It is theorized that four possible domains may be responsible for these results: presence of hypothalamic neuronal subpopulations, role of circadian rhythm, role of hypocretins in locomotive behavior and inextricably confounded variables. These are discussed at length.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Psychology

Year

2004

Author dc:creator

• Dunn, Kelly

Contributors dc:contributor

• Vaccarino, Anthony
• Canavier, Carmen
• LaHoste, Gerald

• Sleep deprivation
• food deprivation
• hypocretins
• orexins
• preprohypocretin

Repository record dc:identifier

https://scholarworks.uno.edu/td/188

OAI identifier oai:identifier

oai:scholarworks.uno.edu:td-1192