Development of 11-plex assay for the rapid screening of samples for detection of Shiga toxin-producing E. coli

Shiga toxin-producing Escherichia coli (STEC) have been identified by the USDA as a serious threat to the nations health stemming from contaminations in the food supply, specifically, the beef chain. I have developed an assay that is able to screen samples for STEC in a rapid and multiplexed format. Multiplex oligonucleotide ligation-PCR (MOL- PCR) is a nucleic acid based assay patented at Los Alamos National Laboratory (LANL) that uses flow cytometry and multiplex microsphere arrays for detection of nucleic acid based signatures. By using MOL-PCR for detecting unique STEC DNA signatures in samples from the beef supply chain (farm to table) this assay will provide a multiplex and high throughput complement to the multiplex PCR assays currently in use. This research is focused on DNA detection of 8 STEC serotypes (STEC-8): O26, O45, O103, O104, O111, O121, O145, and O157:H7 as well as the virulence genes: stx1, stx2, and eae. The goal is to produce a multiplex panel of MOL-PCR probes for identifying DNA signatures corresponding to each of the STEC-8 serotypes and ultimately strain specific identification.'

Name thesis:degree_name

Biomedical Engineering

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biomedical Engineering

Year

2015

Author dc:creator

• Woods, Travis

• MOL-PCR
• Shiga toxin-producing E. coli
• STEC

Language dc:language

English

Identifier

https://digitalrepository.unm.edu/bme_etds/1

OAI identifier oai:identifier

oai:digitalrepository.unm.edu:bme_etds-1000