{"id":{"repo_id":"unm","oai_identifier":"oai:digitalrepository.unm.edu:bme_etds-1000"},"canonical_url":"https://search.dev.ndltd.org/etd/unm/oai:digitalrepository.unm.edu:bme_etds-1000","repository":{"repo_id":"unm","name":"University of New Mexico","base_url":"https://digitalrepository.unm.edu/do/oai/"},"display":{"title":"Development of 11-plex assay for the rapid screening of samples for detection of Shiga toxin-producing E. coli","abstract":"Shiga toxin-producing Escherichia coli (STEC) have been identified by the USDA as a serious threat to the nations health stemming from contaminations in the food supply, specifically, the beef chain. I have developed an assay that is able to screen samples for STEC in a rapid and multiplexed format. Multiplex oligonucleotide ligation-PCR (MOL- PCR) is a nucleic acid based assay patented at Los Alamos National Laboratory (LANL) that uses flow cytometry and multiplex microsphere arrays for detection of nucleic acid based signatures. By using MOL-PCR for detecting unique STEC DNA signatures in samples from the beef supply chain (farm to table) this assay will provide a multiplex and high throughput complement to the multiplex PCR assays currently in use. This research is focused on DNA detection of 8 STEC serotypes (STEC-8): O26, O45, O103, O104, O111, O121, O145, and O157:H7 as well as the virulence genes: stx1, stx2, and eae. The goal is to produce a multiplex panel of MOL-PCR probes for identifying DNA signatures corresponding to each of the STEC-8 serotypes and ultimately strain specific identification.'","abstract_html":"Shiga toxin-producing Escherichia coli (STEC) have been identified by the USDA as a serious threat to the nations health stemming from contaminations in the food supply, specifically, the beef chain. I have developed an assay that is able to screen samples for STEC in a rapid and multiplexed format. Multiplex oligonucleotide ligation-PCR (MOL- PCR) is a nucleic acid based assay patented at Los Alamos National Laboratory (LANL) that uses flow cytometry and multiplex microsphere arrays for detection of nucleic acid based signatures. By using MOL-PCR for detecting unique STEC DNA signatures in samples from the beef supply chain (farm to table) this assay will provide a multiplex and high throughput complement to the multiplex PCR assays currently in use. This research is focused on DNA detection of 8 STEC serotypes (STEC-8): O26, O45, O103, O104, O111, O121, O145, and O157:H7 as well as the virulence genes: stx1, stx2, and eae. The goal is to produce a multiplex panel of MOL-PCR probes for identifying DNA signatures corresponding to each of the STEC-8 serotypes and ultimately strain specific identification.'","abstract_has_math":false,"creators":["Woods, Travis"],"institution":null,"degree_name":"Biomedical Engineering","degree_level":"Thesis","degree_discipline":"Biomedical Engineering","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-11-13T08:00:00Z","date_published":"2015-11-13T08:00:00Z","updated_at":"2026-07-24T05:25:53Z","subjects":["MOL-PCR","Shiga toxin-producing E. coli","STEC"],"languages":["English"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalrepository.unm.edu/bme_etds/1"],"render_values":[{"text":"https://digitalrepository.unm.edu/bme_etds/1","href":"https://digitalrepository.unm.edu/bme_etds/1","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/1928/31697","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Woods, Travis"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biomedical Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis","Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Biomedical Engineering"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["MOL-PCR","Shiga toxin-producing E. coli","STEC"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/1928/31697","https://digitalrepository.unm.edu/bme_etds/1"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Shiga toxin-producing Escherichia coli (STEC) have been identified by the USDA as a serious threat to the nations health stemming from contaminations in the food supply, specifically, the beef chain. I have developed an assay that is able to screen samples for STEC in a rapid and multiplexed format. Multiplex oligonucleotide ligation-PCR (MOL- PCR) is a nucleic acid based assay patented at Los Alamos National Laboratory (LANL) that uses flow cytometry and multiplex microsphere arrays for detection of nucleic acid based signatures. By using MOL-PCR for detecting unique STEC DNA signatures in samples from the beef supply chain (farm to table) this assay will provide a multiplex and high throughput complement to the multiplex PCR assays currently in use. This research is focused on DNA detection of 8 STEC serotypes (STEC-8): O26, O45, O103, O104, O111, O121, O145, and O157:H7 as well as the virulence genes: stx1, stx2, and eae. The goal is to produce a multiplex panel of MOL-PCR probes for identifying DNA signatures corresponding to each of the STEC-8 serotypes and ultimately strain specific identification.'"]},{"key":"dc:title","label":"Title","values":["Development of 11-plex assay for the rapid screening of samples for detection of Shiga toxin-producing E. coli"]}]}],"canonical_facts":{"dc:creator":["Woods, Travis"],"dc:description.abstract":["Shiga toxin-producing Escherichia coli (STEC) have been identified by the USDA as a serious threat to the nations health stemming from contaminations in the food supply, specifically, the beef chain. I have developed an assay that is able to screen samples for STEC in a rapid and multiplexed format. Multiplex oligonucleotide ligation-PCR (MOL- PCR) is a nucleic acid based assay patented at Los Alamos National Laboratory (LANL) that uses flow cytometry and multiplex microsphere arrays for detection of nucleic acid based signatures. By using MOL-PCR for detecting unique STEC DNA signatures in samples from the beef supply chain (farm to table) this assay will provide a multiplex and high throughput complement to the multiplex PCR assays currently in use. This research is focused on DNA detection of 8 STEC serotypes (STEC-8): O26, O45, O103, O104, O111, O121, O145, and O157:H7 as well as the virulence genes: stx1, stx2, and eae. The goal is to produce a multiplex panel of MOL-PCR probes for identifying DNA signatures corresponding to each of the STEC-8 serotypes and ultimately strain specific identification.'"],"dc:identifier":["http://hdl.handle.net/1928/31697","https://digitalrepository.unm.edu/bme_etds/1"],"dc:language":["English"],"dc:subject":["MOL-PCR","Shiga toxin-producing E. coli","STEC"],"dc:title":["Development of 11-plex assay for the rapid screening of samples for detection of Shiga toxin-producing E. coli"],"thesis:degree_discipline":["Biomedical Engineering"],"thesis:degree_level":["Thesis","Masters"],"thesis:degree_name":["Biomedical Engineering"]},"updated_at":"2026-07-24T05:25:53Z"}