Comparison of the sodium ion channel densities, the water flux changes, and the membrane capacitance changes in the pelvic and pectoral skin of the toad, Bufo woodhousei

The amphibian antidiuretic hormone arginine vasotocin (AVT) stimulated osmotic water flow (JH{dollar}\sb2{dollar}O) across isolated skin from the pelvic but not the pectoral region of the toad, Bufo woodhousei. The apical membrane capacitance (C{dollar}\sb{\rm a}{dollar}) of the cells in the stratum granulosum of neither skin region was changed by AVT treatment when an osmotic gradient was present across the tissue; Short circuit current (I{dollar}\sb{\rm sc}{dollar}) and the amiloride sensitive current (I{dollar}\sb{\rm Na\sp+}{dollar}) both increased significantly in the pelvic and in the pectoral skin after AVT treatment, but to a larger degree in the pelvic skin. If M is expressed as a function of C{dollar}\sb{\rm a}{dollar}, the density of Na{dollar}\sp+{dollar} channels (assuming 1{dollar}\mu{dollar}F/cm{dollar}\sp2{dollar}) was 90 channels/{dollar}\mu{dollar}m{dollar}\sp2{dollar} of actual cell membrane area before AVT treatment and 273 channels/{dollar}\mu{dollar}m{dollar}\sp2{dollar} after AVT treatment. In the pectoral skin M did not increase significantly, however, I{dollar}\sb{\rm Na\sp+}{dollar} was linearly related to M. The current through single Na{dollar}\sp+{dollar} channels (i) did not change in either skin region after AVT treatment. (Abstract shortened with permission of author.).

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biology

Grantor dc:publisher

University of Nevada, Las Vegas

Year

1989

Author dc:creator

• Baker, Cathleen Ann

Contributors dc:contributor

• Stanley D. Hillyard

Statement dc:rights

• IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/

Language dc:language

English

Identifier

https://oasis.library.unlv.edu/rtds/43

OAI identifier oai:identifier

oai:oasis.library.unlv.edu:rtds-1042