The heterogeneous biochemical modification of porous collagen scaffolds for tissue engineering applications

The extracellular matrix is a complex and dynamic collection of collagens, non-collagenous matrix proteins, glycosaminoglycans and growth factors. The ECM plays a significant role in guiding the behavior of cells in their tissue specific niche. The recapitulation of both the mechanical and biochemical cues of the ECM is an important objective towards in vitro engineering of functional tissue replacements. Herein I describe my efforts towards the biochemical modification of 3D, porous collagen scaffolds as ECM mimics. Benzophenone mediated photochemistry was used to decorate scaffolds with patterns of vascular endothelial growth factor, which were shown to induce endothelial cells towards a vasculogenic lineage. The use of a two photon excitation for BP patterning was proven affective for surface patterning but ineffective for patterning past a couple hundreds of microns. Heterogenous scaffold patterning in all three dimensions was achieved by collagen slurry layering prior to scaffold freeze drying. A single scaffold was created with two distinct compartment a high modulus “bone” compartment and a low modulus “cartilage” compartment. . This technique was used to model the osteochondral interface.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Turgeon, Aurora

Contributors dc:contributor

• Bailey, Ryan C.
• Harley, Brendan A.
• Murphy, Catherine J.
• Perry, Richard H.

• Photopatterning
• benzophenone
• scaffold

Statement dc:rights

• Copyright 2015 Aurora Turgeon

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/87973

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/87973