From Technology to Chromatin Biology: Contemporary Mass Spectrometry of Histones and Chromatin Associated Proteins

We have also developed a top down-like approach (middle down) which analyzes large peptides to characterize PTM profiles on histone H3. Interestingly, we were able to conclude that methylation and acetylation levels on histone H3 are highly effected by perturbations of DNA methylation. In the past years we have expanded the applications of top down and middle down MS to other chromatin-associated proteins like HMGA1. In this study we were able to link PTM dynamics (phosphorylation of Ser 43) on HMGA1a to DNA repair and the early events in apoptosis.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Siuti, Nertila

Contributors dc:contributor

• Kelleher, Neil L.

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(MiAaPQ)AAI3347511

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/87897