Manipulation of Anthocyanin Accumulation by Vaccinium Pahalae in Vitro

Elicitation of the cells by both biotic (purified beta-glucan and chitosan) and abiotic (FeEDDHA and CuSO4) elicitors resulted in significant enhancement of anthocyanin accumulation. Maximum anthocyanin production using the two most effective elicitors (90 muM FeEDDHA or 10 mg L -1 beta-glucan) was achieved when cultures were treated on day 3 (beta-glucan) or day 0 (FeEDDHA) after the initiation of the cultures. A concentration-dependent response was exhibited by cultures treated with exogenous methyl jasmonate (MJ), a signaling compound. The addition of 0.5 muM MJ alone provoked a striking 2.8 fold increase in anthocyanin production over the control, however, no additive effect on anthocyanin production was observed in any treatments which combined MJ and beta-glucan or FeEDDHA. Conditioning of the cells with a preculture in either MJ, beta-glucan or FeEDDHA similarly did not enhance anthocyanin production. Inoculation of cultures elicited by MJ or beta-glucan with ibuprofen, a reported inhibitor of jasmonate biosynthesis, dramatically stimulated, rather than inhibited, anthocyanin production, resulting in levels of accumulation beyond any of the tested elicitor combinations.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Horticulture

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Fang, Yimin

Contributors dc:contributor

• Mary Ann L. Smith

• Biology, Cell

Language dc:language

eng

Identifier

(MiAaPQ)AAI9944846

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/87830