Biochemical and Molecular Characterizations of a Novel DNA Repair Enzyme, CPD -Photolyase, and Late Promoters of Fowlpox Virus

To further exploit FPV as a vector, characterization of strong promoters were attempted using a lacZ gene based reporter assay. The transcriptional element of ORF P190 appeared to be the strongest. In contrast, the promoter associated with the ORF, P180, was the weakest. Interestingly, a novel bi-directional promoter controlling the expression of ORFs 130 and 131 appeared to be active whose expression was one log fold less than that of the P190 promoter. Subsequently when the critical positions in the spacer and the thymidine after the start codon were substituted with those present in P190, the activity of the weakest promoter was increased to as high as thirty times more than the unaltered promoter, P180. When similar substitutions were made in the strongest promoter, P190, its activity was reduced to 50%. Thus the present study underscores the significance of nucleotides flanking the transcriptional and translational start sites. The promoters identified from this study, P190, P191, P174, as well as the genetically manipulated promoter, P180SG and the bi-directional promoter, P130 and P131 can be used for the expression of foreign genes by FPV vectors.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Veterinary Clinical Medicine

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Srinivasan, Viswanathan

Contributors dc:contributor

• Deoki N. Tripathy

• Health Sciences, Immunology

Language dc:language

eng

Identifier

(MiAaPQ)AAI3070053

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/87118