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University of Illinois at Urbana-Champaign

Characterization and Functional Analysis of the Arabidopsis Intron Splicing Factor ASF/SF2

Abstract

dc:description

ASF/SF2 protein is first time shown to be a potential substrate for the mitotic cdc2/cyclin kinase. UV crosslinkage assay demonstrate RNA binding ability of SF2a and RRM domain, but not SF2b protein. Immunostaining of TN-5 cells expressing recombinant proteins and western blot analyzing cytoplasmic and nuclear fractions of expression cells showed targeting of SF2a, SF2b and their SR domains to the nuclei. SR domain, possibly required phosphorylation, is responsible for the nuclear localization signal.

Degree

thesis:*
Name thesis:degree_name
Ph.D.
Level thesis:degree_level
Dissertation
Discipline thesis:degree_discipline
Plant Biology
Grantor
University of Illinois at Urbana-Champaign
Year dc:date
2015

Author and committee

dc:creator, dc:contributor.*
Author dc:creator
  • Su, Chun-Lin
Contributors dc:contributor
  • Schuler, Mary A.

Subjects

dc:subject × 1

Rights

Language dc:language
eng

Identifiers

dc:identifier.*
Identifier
(MiAaPQ)AAI9904599
OAI identifier oai:identifier
oai:www.ideals.illinois.edu:2142/87064

Chain of custody

source
Harvested from
University of Illinois - Urbana-Champaign
Base URL
www.ideals.illinois.edu/oai-pmh
Last updated
2026-07-22
Source record
OAI-PMH GetRecord
citation

Su, Chun-Lin. Characterization and Functional Analysis of the Arabidopsis Intron Splicing Factor ASF/SF2. Dissertation thesis, University of Illinois at Urbana-Champaign, 2015. http://hdl.handle.net/2142/87064