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University of Illinois at Urbana-Champaign
Identifying the Rubisco Recognition Domain and Critical Arginines in Activase and Engineering Activase With Improved Thermostability for Arabidopsis
Abstract
dc:descriptionHeat sensitivity of Rubisco activase(s) confers a major limitation on leaf photosynthesis at moderately high temperature in Arabidopsis. To overcome this limitation, a thermostable activase for Arabidopsis was generated by engineering the more thermostable tobacco activase with an Arabidopsis Rubisco recognition domain. The resulting chimeric activase displayed an improved thermostability, activated Arabidopsis Rubisco and maintained Rubisco activation state at moderately high temperature in vitro. Transgenic Arabidopsis plants expressing the chimeric activase were created, allowing for further characterization.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Plant Biology
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2015
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Li, Cishan
- Contributors dc:contributor
-
- Archie R. Portis, Jr.
Subjects
dc:subject × 1Rights
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
- (MiAaPQ)AAI3223653
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/87049