Effects of Changes of Redox Properties of the Chloroplast ATP Synthase in Arabidopsis

"Three mutations, designated as C199S, C205S, C199S/C205S, were generated by transformating wild type (Columbia ecotype) plants with the site-directed mutated gene constructs of gamma-subunit. The transgenes were overexpressed behind a constitutive 35S promoter compared with the endogenous gamma-subunit gene. In the isolated mutant chloroplasts, the high ATPase activities did not require DTT treatment while in the wild-type plant, ATPase activities increased after DTT treatment indicating that the cysteine to serine substitutions caused loss of the redox regulation and the ATP synthases were locked in reduced configuration. The ""permanently reduced"" ATP synthases could not be fully deactivated in the dark. There was no significant difference in photosynthesis rate and growth rate suggesting the redox regulation is not very important for plants."

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Plant Biology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Wu, Guosheng

Contributors dc:contributor

• Ort, Donald R.

• Biology, Plant Physiology

Language dc:language

eng

Identifier

(MiAaPQ)AAI3101995

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/87037