Angiotensin II Modulation of NMDA Receptor Function: Characterization of Mechanisms Attenuating Excitotoxicity in Neurons

In the last study, we investigated the involvement of Bcl-2 in the suppression of NMDA-mediated neurotoxicity by Ang II. The viability of differentiated N1E and NG108 neuronal cell lines was reduced following exposure to NMDA in a dose-dependent manner. Excitotoxicity in N1E cells was inhibited by the noncompetitive NMDA receptor antagonist, MK-801. Further, NMDA receptor-mediated cell death was attenuated by Ang II in NG108 cells. A concentration-dependent decrease in intracellular Bcl-2 protein levels was observed following treatment with NMDA, and this reduction was inhibited by MK801. Addition of Ang II suppressed the NMDA receptor-mediated reduction in Bcl-2. The Ang II effect on NMDA-mediated changes in Bcl-2 expression was blocked by PD 123319, but was not significantly altered by losartan. Taken together, these results suggest that the decrease in Bcl-2 expression induced by NMDA is attenuated by AT2 activation. (Abstract shortened by UMI.).

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Schelman, William Russell

Contributors dc:contributor

• Weyhenmeyer, James A.

• Biology, Molecular

Language dc:language

eng

Identifier

(MiAaPQ)AAI9971184

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/86759