The Effects of Four Consecutive Days of Acute Exercise on Macrophage Antigen Presentation

These studies determined the effects of exercise on the ability of M&phis;'s to present antigen to T cells. Pathogen-free male Balb/c mice (8 +/- 2 wk) were randomly assigned to either home cage control (HCC), moderate exercise (MOD, 18 m min-1, 5% grade, 0.5 hr day1), exhaustive exercise (EXH, 18--30 m min-1, 3 hr day), or treadmill control (TC, housed on treadmill for 3 hr but did not exercise) groups. The mice underwent treatments for a period of 4 days during peritoneal thioglycolate inflammation. Peritoneal M&phis;'s were harvested, purified, and incubated with chicken ovalburnin (C-OVA, 0--10 mg mL -1) for 18 hr. M&phis;'s were then co-cultured with C-OVA specific T cells for 48 hr at which time the supernatants were harvested and analyzed via ELISA for IL-2 as an indication of M&phis; AP. EXH exhibited suppressed (25--34 %) M&phis; AP across a wide range of C-OVA doses when measured immediately, 3, and 24 hr post-exercise. In contrast, MOD had reduced M&phis; AP only at 3 hr post-exercise. M&phis; AP was also lower in TC (4--27 %) when compared to HCC, but was significantly higher than EXH. The reduction in M&phis; AP was not due to exercise-induced differences in M&phis; number, percentage, expression of several accessory molecules (i.e., ICAM-1, B7-2 or MHC II), or in vitro production of M&phis; soluble factors (i.e., NO, PGE2, or TGF-beta). However, the exhaustive exercise-induced suppression did result from a reduced ability to generate antigenic peptides. This reduction in the ability of M&phis;s, from exhaustive exercised mice, to generate antigenic peptides may be mediated, in part by an increased lysosomal pH. In conclusion, the data lends evidence that may help explain the increased incidence of infection observed following prolonged exhaustive exercise or over-training.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Kinesiology and Community Health

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Ceddia, Michael Anthony

Contributors dc:contributor

• Woods, Jeffrey A.

• Health Sciences, Recreation

Language dc:language

eng

Identifier

(MiAaPQ)AAI9944812

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/86416