Fluorescent Tools for Watching Conformational Changes of Biological Molecules

Finally, we used a single molecule fluorescence quenching assay to study the timing of the neck linker conformational change that occurs as kinesin walks processively along a microtubule. We observed repetitive, alternating cycles of fluorescence and quenching as individual kinesin motors walked along microtubules. By monitoring the position of the kinesin during these fluorescence cycles, we were able to conclude that one cycle of quenching/unquenching occurs with each 8 nm step, and that each step corresponds to an ATP-induced separation of neck linkers.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biophysics and Computational Biology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Toprak, Erdal

Contributors dc:contributor

• Selvin, Paul R.

• Biophysics, General

Language dc:language

eng

Identifier

(MiAaPQ)AAI3301237

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/85466