In Vitro Effects of Actively Delivered Coencapsulated Curcumin and Cisplatin Nanoliposomes on Squamous Oral Carcinoma

Multidrug carriers were also formulated using a Dipalmitoyl Phosphatidylcholine (DPPC) as a primary lipid, with incorporation of a lysolipid to achieve controlled release of the drugs loaded within. Diagnostic ultrasound energy was then employed as a tool to achieve controlled release from the newly developed multidrug carriers at a frequency of 7.5 MHz and four different power intensities depending on the pulse repetition time. The optimal lipid composition and the intensity settings of ultrasound energy were chosen to formulate a new class of nanoliposomes---ultrasound sensitive nanoliposomes (USNL). The USNLs released increasing amounts of drug in response to increasing irradiation times while the drug release was not significant when the Non-USNLs were exposed to ultrasound energy levels. In vitro studies to test the cytotoxicity of the formulations showed that the USNLs significantly inhibited cell survival of SCC9 oral cancer cells, as compared to the Non-USNLs. We hypothesize that the drug is released due to increased membrane permeability during exposure to ultrasound energy. The lipid composition and energy levels play a key role in determining the efficacy of diagnostic ultrasound energy as a tool for drug delivery. Thus the novel chemotherapeutic approach with the use of compound liposomes, when combined with an active delivery modality can be employed as the primary treatment rather then opting for cosmetically harsh surgery. This may also help in better organ preservation and functioning, which in turn would improve the overall quality of life in patients with these tumors.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Speech and Hearing Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Gosangari, Saujanya Laxmirajam

Contributors dc:contributor

• Kenneth L. Watkin

• Health Sciences, Pharmacy

Language dc:language

eng

Identifier

(MiAaPQ)AAI3337994

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/85223