Biocontrol of Rhizoctonia Solani on Soybean With Bacillus Amyloliquefaciens Producing Iturin A

Three major antifungal compounds produced by strain B94 were purified from the culture filtrate using chromatography techniques including ultrafiltration, SPE, TLC, and HPLC. Amino acid composition analysis showed all the three isolated compounds consist of Asn, Gln, Ser, Pro, and Tyr in a molar ratio of 3:1:1:1:1 in their molecules. FAB MS/MS analysis showed that they belong to iturin A, a group of cyclic lipopeptide antibiotics normally produced by Bacillus subtilis. One compound, with a mass of 1042.5533, was identified as iturin A$\sb2.$ The remaining two compounds are closely related to iturin A$\sb2$, may be iturin A$\sb4$ and A$\sb7,$ respectively. The peptide backbone was opened chemically, and the resulting linear peptide was sequenced using the Edman degradation method. The results are consistent with that of the FAB MS/MS results. This study found that strain B94 of B. amyloliquefaciens produced iturin A.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Yu, Guo-Yun

Contributors dc:contributor

• Sinclair, James B.

• Chemistry, Organic

Language dc:language

eng

Identifier

(MiAaPQ)AAI9904635

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/85055