Modification of Vegetable Soybean Sugar Content via Gene Transformation

To modify soybean sugar content, somatic embryos of soybean cvs. Peterson Jade, Daelip Hakja (PI 157.419), Yakumo Meaka (PI 417.468), Kinshu (PI 549.065) Kaohsiung No.1 (PI 536.546) and Jack were transformed with yeast invertase (SUC 2) and α-galactosidase (agl A) from Aspergillus niger under the control of the lectin promoter using particle bombardment. The transformation was not very successful. In some cultivars, no green clumps were obtained after 8 weeks of selection in FG medium containing 25 μg/ml hygromycin B. The green clumps obtained from transformation and selection were subjected to PCR analysis for the transgenes. Some lines were PCR positive for the hygromycin resistance and/or α-galactosidase genes but Southern and northern analysis gave negative results. These results suggest that the transformed genes were lost during the somatic embryo multiplication due to the friability of some green clumps that were discarded during the culture process.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Chanprame, Sermsiri

Contributors dc:contributor

• Widholm, Jack M.

• Agriculture, Plant Culture

Language dc:language

eng

Identifier

(MiAaPQ)AAI9834660

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/85052