The Effects of Soy and Soy Derived Components on the Growth of Estrogen -Dependent Breast Cancer Tumors

The purpose of the studies presented here was to determine if: (1) the chemical form of genistein affects its ability to stimulate the growth of estrogen-dependent breast cancer tumors, (2) the matrix of the food in which the isoflavone is consumed influences its activity in vivo, and (3) genistein would stimulate the growth of chemically induced mammary tumors in rats. The first study was designed to determine if dietary genistin, the glycoside form of genistein, was capable of stimulating the growth of MCF-7 tumors transplanted into athymic mice. Dietary genistin resulted in increased tumor growth rate and cellular proliferation similar to that observed with genistein. The objective of the second study was to evaluate the ability of various soy products containing genistin, but differing in their content of other biologically active components of soy, to affect the growth of MCF-7 tumors. Soy products investigated included whole soy flour, soy molasses, NovasoyRTM, a mixture of isoflavones, and genistin in pure form. Each of the products was fed so that it would provide equivalent amounts of genistin. MCF-7 tumor growth was stimulated in animals consuming soy molasses, NovasoyRTM, the mixture of isoflavones, and genistin alone. Tumors in mice consuming soy flour with an equal concentration of genistin maintained their original size, but did not grow. Collectively these data suggest that the matrix of the food in which genistein is consumed may impact it's activity. The final study was designed to evaluate how genistein would affect growth of chemically-induced mammary tumors in rats. We found that in ovariectomized rats dietary genistein increased the wet weight of tumors compared to control animals.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Nutritional Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Allred, Clinton David

Contributors dc:contributor

• Helferich, William G.

• Health Sciences, Oncology

Language dc:language

eng

Identifier

(MiAaPQ)AAI3069967

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84931