Selection and Characterization of a Steroid Receptor DNA Binding Domain-Based Repressor of Estrogen Response Element Containing Promoters

To generate a repressor of the ERE-containing genes, we dimerized one of the up-binding mutants, DBD5, with a flexible linker and fused it to a strong KRAB repressor domain. The dimerized FLAGDBD5-KRAB (FdDBD5K) is a much more potent repressor than the monomer FLAGDBD5-KRAB (FDBD5K). The FdDBD5K elicited significant repression of both constitutive and estrogen receptor induced transcription on a cERE containing promoter and on a natural pS2 promoter. The FdDBD5K chimera should be a useful new tool for controlling the transcription of ERE-containing promoters.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Chusacultanachai, Sudsanguan

Contributors dc:contributor

• Shapiro, David J.

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(MiAaPQ)AAI9944821

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84903