Antigen Recognition by an Alloreactive T Lymphocyte Clone

In chapter two, the ability of CTL 2C to recognize thiol-modified peptide variants is analyzed. A method for the synthesis and screening of thiol-modified peptides that are recognized by T cells is presented. Chapter three continues the peptide variant approach with examination of the ability of clone 2C and its receptor to recognize altered peptide ligands involving amino acid substitutions in the center of the alloantigenic peptide. In chapter four, the energy of antigen recognition is mapped using soluble 2C TCR and the alanine-scanning mutagenesis technique. A majority of the binding energy for alloantigen recognition is derived from residues within the CDR 1 and 2 regions of the 2C TCR. Additional TCR mutants are analyzed in chapter five, including several which improve the affinity of the TCR for pMHC. Finally, in chapter six we have examined the ability of 2C TCR transgenic mice on a RAG knockout background to recognize and eliminate tumors bearing the L$\sp{\rm d}$ alloantigen. We show that tumor rejection in this system occurs independent of both B7 costimulatory molecules and CD4$\sp{+}$ helper T cells.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Manning, Thomas Crellin

Contributors dc:contributor

• Kranz, David M.

• Health Sciences, Oncology

Language dc:language

eng

Identifier

(MiAaPQ)AAI9904535

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84893