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University of Illinois at Urbana-Champaign
Human Estrogen Receptor(alpha) Activity on Estrogen Responsive, Integrated Chloramphenicol Acetyltransferase (Cat) Reporter Constructs in Chinese Hamster Ovary (Cho) Cells
Abstract
dc:descriptionThe estrogen receptors endogenous to CHO cells did not induce transcription of chromosomal copies of the more complex 2ERETKCAT promoter. In transient transfections hERα regulated transcription of the three 2ERETKCAT cell lines. We found that an ER mutant containing only AF1 elicited a dose dependent activation of the 2ERETKCAT promoter. This represents the first report of activation of an integrated reporter gene by this activation function.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Biochemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2015
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Eicken, Elizabeth Jean
- Contributors dc:contributor
-
- Shapiro, David J.
Subjects
dc:subject × 1Rights
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
- (MiAaPQ)AAI9834670
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/84890