Engineering T Cell Receptors for Potential Therapeutic Applications

The overall goal of developing new engineering strategies to allow for the isolation of high affinity mutants of many different TCRs was extended to methods for affinity maturation in Chapter 4. Previous affinity-based selections have involved incubating yeast cells with fluorescently labeled recombinant pepMHC ligand. Many pepMHC ligands are difficult to express and purify, so an alternative affinity selection strategy that obviates the need for this material would likewise facilitate affinity maturation. The new method described in Chapter 4 selects for yeast-displayed high-affinity TCR mutants by virtue of their binding to antigen-presenting cells. The concepts and strategies described herein not only provide insight into the folding of TCR domains, but also expand the repertoire of methods for TCR engineering by yeast display.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Richman, Sarah A.

Contributors dc:contributor

• Kranz, David M.

• Biology, Molecular

Language dc:language

eng

Identifier

(MiAaPQ)AAI3290358

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84845