Studies on the Proton Pumping Mechanism of Aa(3)-Type Cytochrome C Oxidase

The monomeric form of bovine heart mitochondrial cytochrome c oxidase was used to detect directly the proton uptake and release events during the F-to-O transition. Absorption change of the pH sensitive dye was monitored spectroscopically. Rapid proton release followed by slow proton uptake was observed. This result indicates that both the pumped protons and the protons required for chemistry at the active site might come from an internal proton pool containing at least two protons.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Han, Dan

Contributors dc:contributor

• Gennis, Robert B.

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(MiAaPQ)AAI3223606

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84824