Regulation and Functionality of Stress-Induced Cytochrome P450 Monooxygenases: A Functional Genomics Approach

Whereas Arabidopsis P450s are typically membrane-associated, the hydrophobic nature of membrane-binding regions oftentimes creates difficulties in the solubilization, isolation and study of these proteins using traditional biochemical techniques. Paralleling the study of individual P450s was the development of a biotechnique to transfer membrane proteins directly from partially fractionated cellular membranes into soluble nanostructures that contain a native-like membrane environment. The resulting system is conducible to many of the biochemical methodologies previously restricted to soluble proteins and adds another tool to aid in the characterizations of this important class of enzymes.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Civjan, Natanya R.

Contributors dc:contributor

• Sligar, Stephen G.

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(MiAaPQ)AAI3198956

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84815