University of Illinois at Urbana-Champaign
Transmembrane Signaling in Bacillus Subtilis Chemotaxis: Determination of the Packing Organization and Structural Changes of McpB in Response to Ligand
Abstract
dc:description"In Chapter 5, we investigated the complementation and activity of a methylesterase phosphorylation point mutant, C-terminal truncated mutant, and a serine active site mutant in vivo to the attractant asparagine to determine the role CheB phosphorylation plays in regulation of receptor demethylation events. Phosphorylation was required for normal methylesterase activity and complementation in vivo; however, enhanced methanol formation occurred briefly without activation of CheB. Therefore, demethylation must involve a hyperactive receptor transition state between ligand free ""relaxed"" states and ligand bound ""adapted"" states. We speculated that the hypersensitive receptor transition is an intrinsic property of receptors which is independent of ""receptor clustering"" or CheB phosphorylation."
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Biochemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2015
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Bunn, Michael Wade
- Contributors dc:contributor
-
- Ordal, George W.
Subjects
dc:subject × 1Rights
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
- (MiAaPQ)AAI3101807
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/84796