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University of Illinois at Urbana-Champaign
Kinetic and Mechanistic Characterization of the Uracil Phosphoribosyltransferase Activity of Bacillus Subtilis PyrR
Abstract
dc:descriptionDirect binding of ligands to PyrR was studied by equilibrium dialysis. The dissociation constant calculated for UMP was 27 +/- 3 muM, whereas the KD for PRPP was 18 +/- 2 muM. There was no detectable binding of uracil or PPi to PyrR, which was consistent with each of the four proposed conformational change models.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Biochemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2015
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Grabner, Gail Keaton
- Contributors dc:contributor
-
- Switzer, Robert L.
Subjects
dc:subject × 1Rights
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
- (MiAaPQ)AAI3070311
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/84785