University of Illinois at Urbana-Champaign
The Membrane Topology of the Microsomal Cytochromes P450: Interfacial Characterization of Protein Structure
Abstract
dc:description"In order to characterize this secondary-binding interaction, a novel surface plasmon resonance technique termed ""analyte gradient-surface plasmon resonance"" (AG-SPR) has been developed and is described here. AG-SPR can be used to rapidly characterize macro-molecular interactions without requiring surface regeneration and has been used to monitor the binding of two truncated P450s with a phosphatidylcholine supported lipid bilayer. The data has been fit to a kinetic model that incorporates a semi-empirical available surface function to account for the effect of steric interactions between pre-adsorbed molecules. The results indicate that P450 does in fact contain a peripheral binding interaction. However, in order to determine an accurate binding affinity, additional factors must be incorporated into the kinetic model."
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Chemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2015
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Retzlaff, Mary Lillian Shank
- Contributors dc:contributor
-
- Sligar, Stephen G.
Subjects
dc:subject × 1Rights
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
- (MiAaPQ)AAI9990124
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/84494