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University of Illinois at Urbana-Champaign
In Vitro Selection of Highly Efficient DNA Enzymes as RNA Nucleases and Metal Biosensors
Abstract
dc:descriptionBased on the superior activity of 17E in Pb2+ than in other metal ions, we evaluated its potential application in sensing Pb 2+ ions. Combining the high selectivity of 17E towards Pb2+ and the high sensitivity of fluorescence detection, 17E can measure the presence of Pb2+ ions over a concentration range of three orders of magnitude, with a detection limit as low as 10 nM. This system represents the first example of DNA-based biosensor for metal ions. Similar approaches can be applied to develop deoxyribozymes that can probe other metal ions and at various concentration ranges.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Chemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2015
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Li, Jing
- Contributors dc:contributor
-
- Lu, Yi
Subjects
dc:subject × 1Rights
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
- (MiAaPQ)AAI9990060
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/84486