University of Illinois at Urbana-Champaign
Studies of the Catalytic-Metal-Binding Site in the Hammerhead Ribozyme Using the Phosphorothioate Approach
Abstract
dc:descriptionThe phosphorothioate approach has been used widely in the literature in combination with activity assays to establish an interaction of metal ions with phosphate oxygens in the ribozyme structure. An enhancement in cleavage rate upon addition of a softer metal ion than Mg2+, such as Mn2+ is taken as evidence for coordination of the metal ion to the phosphorothioate sulfur and by analogy, an interaction of Mg 2+ with oxygen in the native ribozyme. UV-vis, EXAFS and 31 P NMR evidence for the interaction of Hg2+ with the phosphorothioate sulfur in the hammerhead ribozyme is presented here along with evidence for the unexpected discovery that soft metal ions like Hg 2+ and Mn2+ are capable of catalyzing desulfurization of the phosphorothioate at rates similar to that of ribozyme-catalyzed cleavage.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Chemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2015
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Cunningham, Lynette Anne
- Contributors dc:contributor
-
- Lu, Yi
Subjects
dc:subject × 1Rights
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
- (MiAaPQ)AAI9952998
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/84452