Metal Thiolate and Metal Sufide Clusters in Proteins

Finally efforts towards the design of a biosynthetic model of Cu Z from N2OR are reported. The CuZ cluster is the first and only report to date of a copper-sulfide cluster in Nature. It fulfills a catalytic role in N2OR, performing the reduction of N2O to N2 and H2O during anaerobic respiration, with reducing equivalents provided by a CuA site. The cluster is composed of four copper atoms bridged by a central sulfide, with remaining all histidine coordination to the copper atoms. A scaffold protein was selected, and residues were chosen for mutagenesis to create a similar ligand set to CuZ in N2OR. The copper binding properties and copper/sulfide binding properties of the biosynthetic and native N2OR systems were investigated towards efforts to reconstitute them.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Savelieff, Masha Georges

Contributors dc:contributor

• Lu, Yi

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(MiAaPQ)AAI3337915

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84319