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University of Illinois at Urbana-Champaign
The Utility of 7-Deazaguanine: Part I. High Affinity, High Fidelity Modules for Supramolecular Chemistry, And, Part II. Molecular Recognition of Uracil and Thymine
Abstract
dc:descriptionA water-soluble analogue of the 2-naphthyl-7-DeG host was synthesized and evaluated as a molecular recognition unit to a thymine (T) bulge of a short 10-bp oligomer. The ligand was found to stabilize the T bulge quite significantly (+7.8°C) relative to the C bulge (3.6°C) and A and G bulges (≤ 2.0°C) at 5.0 equivalents, when the bulged base was flanked by guanosines (G's). Furthermore, the ligand did not stabilize the normal duplex under any condition. Control studies suggest that the ligand binds C primarily by intercalation, but binds T by intercalation and hydrogen-bonding. Isothermal titration calorimetry experiments gave a K d of 5.4 +/- 0.6 muM to the T bulge.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Chemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2015
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Ong, Hugo Calvin
- Contributors dc:contributor
-
- Zimmerman, Steven C.
Subjects
dc:subject × 1Rights
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
- (MiAaPQ)AAI3290341
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/84277