Development of High Pressure Surface Plasmon Resonance for the Study of Biosystems

Through an initial set of experiments high pressure SPR (HP-SPR) was used to measure changes in the reflectivity of various liquids under hydrostatic pressures ranging from ambient to 276 bar (4000 psi). The changes in refractive index as a function of increasing pressure for these solutions were compared and an indication of how these methods can be used to determine volume changes in biomolecular interactions is provided. The application of HP-SPR was extended to the use of commercially available sensor chips. We have shown that BIAcore(TM) SA chips maintain their surface binding functionality after pressure exposure and that effects to the surface are reversible to 6000 psi. Three test systems were chosen to be studied using this instrument, each having different characteristics; cytochrome c association to cytochrome b5, unfolding of staphylococcal nuclease, and binding of the BamH1 restriction enzyme to its DNA recognition site.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Kelley, Jennifer Gann

Contributors dc:contributor

• Sligar, Stephen G.

• Chemistry, Analytical

Language dc:language

eng

Identifier

(MiAaPQ)AAI3153349

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84153